Thermal Denaturation of Proteins for SDS-PAGE Analysis by Microwave Irradiation

Thermal denaturation of proteins for SDS-PAGE analysis by microwave irradiation.

One of the most useful tools available to researchers is sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (3). It is a rapid and relatively simple method for resolving proteins and is routinely used to monitor proteins during purification, assess expression levels, estimate molecular weights and characterize multimeric proteins. A number of variations exist, but the method d...

Denaturation of proteins by SDS and tetraalkylammonium dodecyl sulfates.

This article describes the use of capillary electrophoresis (CE) to examine the influence of different cations (C(+); C(+) = Na(+) and tetra-n-alkylammonium, NR(4)(+), where R = Me, Et, Pr, and Bu) on the rates of denaturation of bovine carbonic anhydrase II (BCA) in the presence of anionic surfactant dodecylsulfate (DS(-)). An analysis of the denaturation of BCA in solutions of Na(+)DS(-) and ...

Identification of electrophoretic proteins pattern of Fusaium verticillioides isolates from maize by SDS-PAGE.

SDS-PAGE analysis of urinary proteins in dogs with heartworm disease

The aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.Urine samples from 15 heartworm-infected and 15 healthy dogs were taken. Urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (U P/C) ratio were determined. Urine proteins werefractionated using SDS-PAGE. Results showed statistically significant differences ...

Study on pattern of Neospora caninumtachyzoite proteins by SDS-PAGE and Western blotting in aborted cows

The intracellular parasite Neospora caninumis prevalent in several countries and is increasingly recognized as an important cause of abortion and stillbirth in cattle. For characterizeing the tachyzoite antigens of Neospora caninumin aborted cows, sera were obtained from 116 cows which were aborted in the third semester of the pregnancy period and had antibodies to Neospora caninumin ELISA. To ...